The Definitive Guide to cytometers
The Definitive Guide to cytometers
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Details-enriched characterization of mobile populations. Morphological data from photos can increase on the richness of flow cytometry info. By way of example, the determine exhibits an normally common apoptosis assay using Annexin V and PI, including cell imaging to characterize cells in Every single inhabitants to expose morphologically unique capabilities. These insights could not have already been acquired from multiplex staining by yourself.
As samples are obtained about the Attune CytPix Flow Cytometer, the large-velocity brightfield camera captures and shops photos of detected gatherings at a fee around six,000 visuals per 2nd, with regards to the flow rate and graphic dimensions. For higher adaptability, Attune Cytometric Application enables you to modify the impression capture frequency as necessary and choose a specific gate for graphic seize, if demanded.
(B) Uncooked 264.seven macrophages in many cases are annotated inaccurately with respect to particle count because of their complexity and noncircular borders. The quantity of singlets readily available for analysis will increase as soon as the user trains and employs a far more precise design.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a substantial-pace brightfield digicam for simultaneous large throughput flow cytometry and high resolution brightfield imaging.
*Pricing is indicative only and is also topic to change. Make sure you Get in touch with your Agilent representative with any issues. The knowledge established forth herein is not really a suggestion or simply a motivation, both of which are issue to credit score approval, conditions and terms, and availability with Agilent. Agilent Fiscal Solutions is accessible in chosen international locations.
Moreover, it really is illuminating to view the amount of doublets slip by standard singlet gates, and great to confirm the standing of surprising double-beneficial gatherings in my Examination."
Pair your Attune flow cytometer using an autosampler for improved workflow performance, and increase robotic integration for maximum throughput.
Our experts will layout panels for you personally as often times you need—cost-free, no merchandise order essential.
The Attune NxT cytometer is definitely the reliable workhorse instrument your lab can depend upon for constant and trustworthy performance. The Attune CytPix product incorporates a higher-pace brightfield digital camera and AI-pushed impression analysis application, with the latest updates including device learning applications and an intuitive consumer interface, enabling Improved knowledge precision, accuracy, and faster insights into sample biology.
Maybe most significantly, these activities incorporate cells of Evidently unique phenotypes which may lead to incorrect conclusions concerning double optimistic events (especially in rare populations).
Audio waves permit exact cell alignment, leading to Excellent knowledge high quality and ~10X speedier sample flow charges.
Application Take note: Automatic picture analysis cuts down consumer-to-person variability in flow cytometry gating procedures
Leverage the power of advanced technological innovation and innovation to accelerate your discoveries. Have faith in during the Attune Flow Cytometer spouse and children to provide the trustworthiness, speed, and accuracy that set your get the job done aside.
No clean/no lyse leukocyte Evaluation with imaging. Cells ended up acquired from 24-hour-outdated blood by dilution in one mM EDTA ( Professional UX/UI website allows buyers to teach AI designs for expanded apps. (A) The “Teach Design” feature gives annotation applications and authentic-time product education accuracy.
Gating on these prolonged parameters enables you to speedily and properly discover populations of fascination and confirm gating technique with little or no handbook review. The Attune software program offers access to condition-of-the-art equipment Finding out Examination instruments within an expertly designed consumer interface, making it extremely convenient to use.
The Attune Xenith cytometer is often a large-parameter spectral analyzer, delivering each spectral unmixing and conventional flow abilities for thorough and multipurpose cell analysis.
The Attune CytPix Flow Cytometer allows people to visualise samples for the duration of acquisition, confirm the accuracy of gating approaches, and aid results with reputable documentation for publication uses.
What appeared to be a homogeneous population is really additional numerous—an insight that should be regarded as when interpreting success.